HEK293 Media

​Human embryonic kidney (HEK 293) cells and human cell lines are commonly used for Gene Therapy applications (AdenoAssociated Virus) , Gene Modified Cell therapy (Lentivirus), Viral vector Vaccines( Adenovirus) and Proteins production.

• suitable for HEK (e.g., HEK293) and other human cell lines for Infection & Stable expression in suspension
• chemically defined, serum-free, animal component-free, serum-free and hydrolysate-free
• little or no adaptation required from other serum-free media
• supports stable growth of suspension cells at high viability in seed train culture
• manufactured under ISO 9001 QMS (Quality Statement)

Available products

HEK GM media

• complete chemically defined, animal-component-free, serum-free and hydrolysate-free medium
• developed for high-performance Infection of HEK293 and other human cell lines
• supports cell growth and production of recombinant proteins and antibodies
• contains a high level of nutrients and growth factors
• applications: research or in manufacturing applications and for all steps such as thawing, expanding and producing steps can be performed in the same medium, regardless of the production scale
• available volumes: 1 L, 5 L, 10 L, 20 L, 50 L, 100 L and 500 L. 

HEK TF media

• chemically defined, animal-component-free, serum free and hydrolysate-free medium
• developed for cultivation of HEK293 and other mammalian cell lines
• special focus on transfection applications and virus production (e.g. AAV production and lentivirus production) in suspension
• perfect choice for your gene therapy applications
• suited for transient transfection with e.g. polycationic transfection reagents such as polyethylenimine (PEI)
• contains a medium level of nutrients and growth factors
• available volumes: 1 L, 5 L, 10 L, 50 L, 100 L i 500 L. 

HEK ViP NB media

• chemically defined, animal-component-free, serum free and hydrolysate-free medium
• developed for cultivation of HEK293 and other mammalian cell lines
• special focus on transfection applications and virus production (e.g. AAV production and lentivirus production) in suspension
• medium is especially suited for transient transfection with e.g. polycationic transfection reagents such as polyethylenimine (PEI)
• applications: research or in manufacturing applications and all steps such as thawing, expanding, pre-complexing DNA, transfecting and producing steps can be performed in the same medium, regardless of the production scale
• available volumes: 1 L, 10 L, 50 L, 100 L, 150 L i 500 L

HEK ViP NX media

• suitable for HEK (e.g., HEK293) and other human cell lines for Infection & Stable expression in suspension
• chemically defined, serum-free, animal component-free, serum-free and hydrolysate-free
• little or no adaptation required from other serum-free media
• manufactured under ISO 9001 QMS (Quality Statement)
• available volumes: 1 L, 10 L, 20 L, 50 L i 200 L

HEK FS media

• chemically defined, animal component-free cell culture feed supplement
• developed for the use as feeding solution in combination with HEK293 cell culture media, such as HEK GM for recombinant protein production or HEK ViP NX for viral vector production
• the feed supplement contains highly concentrated nutrients to increase the productivity of HEK and other human cells
• it does not contain lipids, hydrolysates, or growth factors
• supports superior production of recombinant proteins and antibodies in suspension culture by maintaining and extending the production capability of HEK cultures
• available volumes: 1 L, 5 L, 10 L, 20 L i 50 L