Minisart® syringe filters
Wide range of filters for sterile filtration
Sartorius Minisart® syringe filters are used for a wide range of applications, including sterile filtration. Our filters are clean and safe as they contain almost no leachables and extractables and possess ultra-low absorption properties. Minisart® acrylic housing filters (MBS) are the perfect choice for sterile filtration and clarification of additives, buffers, reagents, drugs and gases.
Sartolab® RF | BT units for vacuum filtration
Convenient sterile vacuum filtration of cell culture, media, serums and buffers
- highest flow rates with a large filtration surface
- no loss of protein due to a low-protein affinity membrane
- low hold-up volume due to optimized membrane support
- can be used as a stand-alone unit or with the Sartolab® Multistation unit, which allows parallel filtration of up to six samples
Sartolab® P20 & Sartolab® P20 Plus
Fast sterile filtration of aqueous liquids & cell culture media
- highest flow rates with a large surface of filtration (20 cm²)
- no loss of protein with a low-binding membrane
- low hold-up volume due to optimized membrane support
- versions available with prefilter for high particle load solutions
Faq about sterile filtration
We have established a correlation between bubble point and membrane integrity, ensuring reliable sterile filtration. You can follow the methods in the following video to test your Minisart® syringe filters with a hand-held bubble point measurement.
Non-sterile Minisart® types must be sterilized before sterile filtration:
- non-sterile Minisart® High-Flow, NML and NML Plus can be sterilized by ethylene oxide (EO) or by gamma irradiation
- non-sterile Minisart® HY can be sterilized by ethylene oxide (EO)
- non-sterile Minisart® NY, SRP and PES- can be autoclaved or sterilized by ethylene oxide (EO)
- do not sterilize non-sterile Minisart® syringe filters in the pouch and carton of the bulk pack
- sterile Minisart® products cannot be sterilized a second time
The non-sterile Sartolab® P20 can be sterilized by autoclaving or EtO sterilization.
Sterile filtration is performed using microporous membranes filters with a pore size of 0.2 | 0.22 µm or smaller like 0.1 μm.
To obtain a sterile filtrate, we recommend using a sterilizing grade filter with a pore size of 0.2 µm. A sterilizing filter should be capable to withstand a bacterial challenge to retain a minimum of 107 cfu/cm² of B. dimunita.
Microporous membranes with rated pore sizes of 0.2 and 0.22 µm are defined by the FDA as sterilizing filters as they retain the organism Pseudomomas diminuta on the basis of a minimum of 1 x 107 colony forming units (cfu) per cm² of effective filtration area. Therefore, you can consider both as identical if certified for this retention rate.
